A Global Leading Company in Antibody Therapeutics

Products & Services Hybridoma antibody sequence recovery and recombinant antibody production

We provide recombinant antibody production service from customer’s hybridoma cell with mouse or human backbone using HEK293 or CHO cells for the early stage of therapeutic antibody development

Hybridoma 항체 서열 복원 및 재조합 항체 생산
Service includes Delivery package
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    Cloning and sequencing of variable heavy chain and light chain from hybridoma

VH, VL sequence, T-vector
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    Cloning and sequencing of variable heavy chain and light chain from hybridoma

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    Recombinant antibody production (>0.5 mg)

VH, VL sequence, T-vector,
Purified chimeric antibody
Hybridoma 항체 서열 복원 및 재조합 항체 생산
Technology Chimeric antibody design, Expression vector construction, Transient transfection, Serum-free cell culture, Affinity purification
Host cell HEK293 or CHO cell
Time line

2-4 weeks from gene sequence to antibody delivery

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    1 weeks for gene cloning and sequencing

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    1 weeks for expression vector cloning and plasmid production

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    2 weeks for transient expression, antibody purification

Deliverable Purified antibodies and/or DNA sequence in commercial T-vector
Quality
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    > 95% antibody purity by SDS PAGE or Agilent 2100 Bioanalyzer, Protein 230 Kit

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    endotoxin level < 0.2 EU/ug

Application Antibody engineering (light chain shuffling)