You can expect high therapeutic effect when you transfer the CDR(Complementary-Determining Region) from the variable region of therapeutically effective mouse-derived monoclonal antibody into human antibody backbone due to the minimized immunogenicity.
Outline of Humanization by CDR-Grafting
- Mouse hybridoma
- Cloning and sequencing of VH and VL
- Identification of CDRs from VH and VL
- Grafting of CDRs onto human germline VH and VL
- Transient expression and affinity measurement of CDR-grafted humanized antibodies
- Selection of CDR-grafted humanized antibody